Intracellular Antioxidant Activity and Inhibition of Bee Pollens on the Production of Inflammatory Mediators
Curr Dev Nutr. 2019 Jun 13;3(Suppl 1). pii: nzz031.P06-081-19
The purpose of this study is to present scientific evidences for health promotion of bee pollens through the investigation of the antioxidant and the anti-inflammatory effect.
The intracellular antioxidant activity of bee pollen ethanol extracts was investigated by DCFH-DA assay for the oxidative stress induced by lipopolysaccharide (LPS) in mitochondria of macrophages. Inhibition of nitric oxide (NO) and prostaglandin E2 (PGE2) production were confirmed by the addition of bee pollen extracts. Inhibition of transcription/expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) and the inflammatory mediators-production were investigated by adding the bee pollen extracts.
Intracellular oxidative stress by LPS was inhibited at approximately 53% by 0.25 and 3 mg/ml of the Darae-bee pollen extract. It was found that the Acorn- and Mixed bee pollen extract (3 mg/ml) reduced the intracellular oxidative stress at approximately 26.3 and 41.3%, respectively. For anti-inflammatory activity, all three bee pollen extracts (3 g/ml) inhibited effectively the production of NO to an equal to or rather lower than basal level in macrophages. Bee pollen extracts decreased the LPS-induced PGE2 production depending on the amount added, regardless of the kind of bee pollen added. Gene transcription and expression of iNOS and COX-2, an enzyme producing NO and PGE2, respectively, was suppressed by bee pollen extracts and confirmed by RT-PCR and western blot. Additionally, the production of IL-1b, IL-6 and TNF-α, which are inflammatory mediators in macrophages, were inhibited by Darae-, Acorn- and Mixed bee pollen extract.
The ethanol extracts of Darae-, Acorn- and Mixed bee pollen were verified to have the intracellular antioxidant activity and the inhibitory activity on the production of several anti-inflammatory mediators in macrophage cells.
The information contained on these Web pages is not intended to be a substitute for medical evaluation and treatment by a competent, licensed personal health care professional. The American Apitherapy Society, Inc. specifically disclaims any liability
- Honey Has Protective Effect in Cerebrovascular Diseases Липень 25, 2023Apitherapy in Post-Ischemic Brain Neurodegeneration of Alzheimer’s Disease Proteinopathy: Focus on Honey and Its Flavonoids and Phenolic AcidsMolecules 2023, 28(15), 5624Neurodegeneration of the brain after ischemia is a major cause of severe, long-term disability, dementia, and mortality, which is a global problem. These phenomena are attributed to excitotoxicity, changes in the blood–brain
The American Apitherapy Blog
Terms & Conditions